Quantification of peptides in human synovial fluid using liquid chromatography-tandem mass spectrometry.

Quantification of peptides in human synovial fluid using liquid chromatography-tandem mass spectrometry. Talanta. 2018 Aug 15;186:124-132 Authors: Garcia-Ac A, Duy SV, Sauvé S, Moldovan F, Roullin VG, Banquy X Abstract A method to explore the stability of two anti-inflammatory peptides in human synovial fluid (HSF) has been developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The two peptides are BQ123 Cyclo(-D-Trp-D-Asp-L-Pro-D-Val-L-Leu, Mw = 610.7) and R-954 (AcOrn[Oic2, (αMe)Phe5, DβNal7, Ile8]desArg9-bradykinin, Mw = 1194.4). Human synovial fluid samples were analyzed after a protein precipitation step with acetonitrile and dilution with mobile phase. DMSO was used as anti-adsorptive agent. We used an octyl silane column with formic acid (0.1%, v/v) in water as the aqueous mobile phase and acetonitrile isopropanol-formic acid (20:80, 0.1 v/v) as the organic mobile phase and 0.7 mL/min flow rate. The peptides CY-771 and pepstatin A were used as internal standards. Selective detection was performed by tandem mass spectrometry with a heated electrospray source (HESI), operated in positive ionization mode and in selected reaction monitoring acquisition (SRM). The method limit of quantification (injection volume = 10 µL) was 0.17 ng and 1.2 ng, corresponding to 28 and 102 nmol L-1 for BQ123 and R-954 respectively in human synovial fluid. Calibration curves obtained us...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research