GSE111557 Expression data from surface airway epithelial basal cells, ciliated cells, and club cells.

Contributors : J F Engelhardt ; T J LynchSeries Type : Expression profiling by arrayOrganism : Mus musculusTo identify key genes that define surface airway epithelial (SAE) basal cells, we FACS isolated basal, ciliated, and club cell populations as previously reported (Zhao et al., 2014; PMID: 25043474) and performed microarray analysis on isolated mRNA. For fractionating SAE into basal, club, and ciliated populations, cells were stained with EpCAM-PECy7 (eBiosciences), GSI β4-FITC (Sigma), SSEA1-Alexa Fluor® 647 (BioLegend), and CD24-PE (BD Pharmingen) for 30 minutes on ice as previously described (Zhao et al., 2014), prior to FACS. Basal cells were considered EpCAM+ and GSIβ4+. Secretory cells were considered EpCAM+ and SSEA1+. Ciliated cells were considered EpCAM +, GSIβ4- and CD24+.Primary SAE cells were harvested from C57BL/6 mice and sorted into basal, ciliated, and club cell populations for the purpose of identifying enrichment of transcripts specific to each cell type population.

Source: GEO: Gene Expression Omnibus - April 30, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research