A two dimensional metal-organic framework nanosheets-based fluorescence resonance energy transfer aptasensor with circular strand-replacement DNA polymerization target-triggered amplification strategy for homogenous detection of antibiotics.

A two dimensional metal-organic framework nanosheets-based fluorescence resonance energy transfer aptasensor with circular strand-replacement DNA polymerization target-triggered amplification strategy for homogenous detection of antibiotics.
Anal Chim Acta. 2018 Aug 22;1020:1-8
Authors: Yang Q, Zhou L, Wu YX, Zhang K, Cao Y, Zhou Y, Wu D, Hu F, Gan N
Abstract
In the study, a novel two dimensional metal-organic framework (Cu-TCPP nanosheets) based fluorescence resonance energy transfer (FRET) aptasensing platform was developed for detecting antibiotics. Cu-TCPP nanosheets were employed for quenching the background fluorescence and circular strand-replacement DNA polymerization (CSRP) for signal amplification. To fulfill the purpose, we designed an aptamer hairpin probe (HP) whose stem can be opened while specifically binding to target. Then the opened HP would bind with the primer. Under the action of polymerase, extension reaction was induced to generate double-stranded DNA (dsDNA), and then the target was released for the next cycle. Finally, SYBR Green I (SG) can bind with dsDNA to produce strong fluorescence response for quantification of target. It's worth mentioning that the fluorescence of HP/SG complex and free SG could be completely quenched by Cu-TCPP nanosheets while that of dsDNA/SG complex wouldn't be affected. Thus, the sensor produced negligible background signals. It can produce 7.5-fold improved S/N compared to a grap...

Source: Analytica Chimica Acta - April 17, 2018 Category: Chemistry Authors: Yang Q, Zhou L, Wu YX, Zhang K, Cao Y, Zhou Y, Wu D, Hu F, Gan N Tags: Anal Chim Acta Source Type: research