Expression of genes in primo vasculature floating in lymphatic endothelium under lipopolysaccharide and acupuncture electric stimulation

Publication date: Available online 4 April 2018 Source:Journal of Acupuncture and Meridian Studies Author(s): Jun-Young Shin, Jong-Ok Ji, Da-Woon Choi, Sang-Heon Choi, Jong-Gu Choi, Min-Suk Rho, Ji Yoon Lee, Sang-Suk Lee It is known that the existence of primo vascular system (PVS) including the primo nodes and vessels. However, it is poorly understood the relevant genes in the PVS system both patho- and physiologic condition. Here, we first examined the gene expression in primo vessels (PV) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LV) containing PVS. To investigate therapeutic effects, both PV and LV containing PVS were isolated after lipopolysaccharides (LPS) injection and acupuncture electric stimulation (AES) at two acupoints of Joksamni (ST36) and Hapgok (LI04) following LPS. We used reverse transcriptase-polymerase chain reaction (RT-PCR) to examine expression of lymphatic endothelial cell (LEC) markers and inflammatory related genes. We found that LEC markers such as Flt4, Lyve-1, Prox-1, and Pdpn were highly expressed in PV, compared to that of lymphatic endothelium, suggesting pivotal roles of PV in LV under inflammation. Furthermore, lymphatic related genes including Mtf2, Hif1a, Agtr1, and Agtr2 were also overall increased in PV and remarkably increased and these genes except Pparg after AES in two acupoints, implying central role of PV by gene activation.
Source: Journal of Acupuncture and Meridian Studies - Category: Complementary Medicine Source Type: research