Rapid determination of neomycin in biological samples using fluorescent sensor based on quantum dots with doubly selective binding sites

Publication date: 30 May 2018 Source:Journal of Pharmaceutical and Biomedical Analysis, Volume 154 Author(s): Ying-chun Wan, Yan-jie Liu, Chen Liu, Hui-ting Ma, Hui-fang Yu, Jia-wei Kang, Chen-long Gao, Zhong-qiao Wu, Dan Zheng, Bin Lu A new analytical method was developed to detect neomycin in complex biological samples using molecularly imprinted polymer to construct an optical sensor. Fluorescent neomycin-imprinted polymers (fMIPs) containing both imprinted cavity and boronate affinity site were synthesized on the surface of silica-modified quantum dots. The fMIPs exhibited high selectivity to neomycin by having two binding sites for the target analyte. Neomycin analogues (competing for imprinted cavity) and D-glucose (competing for the boronate affinity site) did not affect the selectivity of the fMIPs. When combined with a fluorescent microplate reader, the obtained fMIP sensor displayed a linear concentration-dependent fluorescence quenching in response to neomycin in the range of 2–1000 μg/L, with a limit of detection as 0.16 μg/L. The fMIP sensor was able to detect trace neomycin in biological samples accurately after simple sample pre-treatment. The sensitivity of the fMIP sensor was higher than HPLC equipped with a fluorescence detector. The fMIP sensor containing the doubly selective binding sites provides a selective, sensitive, accurate, and high through-put approach for neomycin monitoring. Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research