Drug Resistance-Associated Mutations in Antiretroviral Treatment-Experienced Patients in Kuwait

Objectives: To investigate the prevalence of non-polymorphic resistance-associated mutations (RAMs) in HIV-1 patients on first-line antiretroviral therapy in Kuwait. Subjects and Methods: Total ribonucleic acid (RNA) was isolated from plasma samples of 42 patients who received first-line non-nucleoside reverse transcriptase inhibitor (NNRTI)-based regimen. HIV-1 protease and reverse transcriptase genetic regions were then amplified by nested reverse-transcription polymerase chain reaction (RT-PCR), and directly sequenced. HIV-1 subtype was identified using the Bayesian phylogenetic method, and resistance-associated mutations were identified using the Stanford University genotypic resistance interpretation algorithm. Results: The HIV-1 viral load at sampling ranged from
Source: Medical Principles and Practice - Category: Internal Medicine Source Type: research