Increased high mobility group A 2 expression promotes transition of cervical intraepithelial neoplasm into cervical cancer.

Increased high mobility group A 2 expression promotes transition of cervical intraepithelial neoplasm into cervical cancer. Oncotarget. 2018 Jan 30;9(8):7891-7901 Authors: Wang L, Shen H, Zhu D, Feng B, Yu L, Tian X, Ren C, Gao C, Li X, Ma D, Hu Z, Wang H Abstract Integration of the high risk human papillomavirus (HR-HPV) genome into host chromatin is an important step in cervical carcinogenesis. We identified HR-HPV integration sites within the human genome through detection of integrated papillomavirus sequences-PCR and assessed the role of high mobility group A 2 (HMGA2) in cervical carcinogenesis in clinical samples and cell lines. HPV integration sites were analyzed in 40 cervical cancer samples, while copy number variation and protein expression were assessed in 19 normal cervixes, 49 cervical intraepithelial neoplasia (CIN), and 52 cervical cancer samples. Overall, 25 HR-HPV integrating loci were detected in 24 cervical samples; HMGA2 was the only recurring integration site. Both HPV copy number and HMGA2 protein expression were higher in cervical cancer than CIN samples. Area under the curve (AUC) values for HMGA2 expression and HPV copy number were 0.910 (95% CI: 0.844-0.976) and 0.848 (95% CI: 0.772-0.923), respectively. Expression of Bcl-2 and Caspase 3 can indicate the cell proliferation and apoptosis. Transfection of HMGA2 siRNA decreased HMGA2 mRNA and protein expression, Bcl-2 expression, inhibited cell proliferation, ...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research