Experimental infection model for vibriosis in Dover sole ( Solea solea ) larvae as an aid in studying its pathogenesis and alternative treatments

AbstractSevere economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The present study aimed at developing a challenge model for vibriosis in Dover sole larvae and testing its applicability to study the effect of the probiotic treatment. For that purpose, larvae were challenged at 10  days post hatching withVibrio anguillarum WT,V. anguillarum HI610 orV. harveyi WT. Following administration ofV. anguillarum WT via immersion at 1  × 107 colony forming units/mL, a larval mortality of 50% was observed at 17  days post-inoculation. In a next step, the probiotic potential of 371 isolates retrieved from Dover sole was assessed by screening for their inhibitory effects againstVibrio spp. and absence of haemolytic activity. One remaining isolate (V. proteolyticus) andV. lentus, known for its protective characteristics in seabass larvae, were further tested in vivo by means of the pinpointed experimental infection model. Neither isolate provided via the water or feed proved to be protective for the Dover sole larvae against challenge withV. anguillarum WT. This developed challenge model constitutes a firm basis to expedite basic and applied research regarding the pathogenesis and treatment of vibriosis as well as for studying the impact of (a)biotic com...
Source: Veterinary Research - Category: Veterinary Research Source Type: research