GSE97775 Two distinct transcription termination modes dictated by promoters

Contributors : Takashi S Miki ; Sarah H Carl ; Helge GrosshansSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Caenorhabditis elegansTranscription termination determines the ends of transcriptional units and thereby ensures integrity of gene regulation. Here we perform genome-wide investigation of RNA polymerase II (Pol II) transcription termination in Caenorhabditis elegans and observe two distinct modes of termination. Whereas a subset of genes requires the exoribonuclease XRN2, a known termination factor, termination of other genes appears independent of, and refractory to, XRN2. Unexpectedly, promoters instruct the choice of termination mode, but XRN2-independent termination additionally requires a compatible region downstream of the 3 ’ end cleavage site. Hence, different termination mechanisms may affect different configurations of Pol II complexes dictated by promoters.

Source: GEO: Gene Expression Omnibus - February 5, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Caenorhabditis elegans Source Type: research