Ionic liquid based ultrasound assisted dispersive liquid-liquid micro-extraction for simultaneous determination of 15 neurotransmitters in rat brain, plasma and cell samples.

Ionic liquid based ultrasound assisted dispersive liquid-liquid micro-extraction for simultaneous determination of 15 neurotransmitters in rat brain, plasma and cell samples.
Anal Chim Acta. 2018 Apr 16;1005:43-53
Authors: Jha RR, Singh C, Pant AB, Patel DK
Abstract
Neurotransmitters are signaling molecules which play a key role in the central nervous system allowing signal transmission in the neuronal synapses. The role of these compounds is very crucial in the biological systems. A rapid, sensitive, economical and derivatisation free method has been developed for the analysis of 15 neurotransmitters in a single run on liquid chromatography tandem mass spectrometry. These 15 neurotransmitters are categorized into 5 groups on the basis of their fragmentation pattern. The proposed method "ionic liquid based ultrasound assisted dispersive liquid-liquid microextraction" hyphenated with tandem mass spectrometry is the first report for the analysis of neurotransmitters in cell samples along with other two matrices (rat brain and plasma). All the parameters that influence the extraction efficiency are optimized with aid of response surface methodology and desirability profile. Under these optimized conditions the developed method has been validated. The limit of detection was in the range of (1) 0.021-0.912 μg L-1 for rat brain samples, (2) 0.028-0.978 μg L-1 for plasma samples and (3) 0.025-0.945 μg L-1 for cell samples wit...

Source: Analytica Chimica Acta - February 2, 2018 Category: Chemistry Authors: Jha RR, Singh C, Pant AB, Patel DK Tags: Anal Chim Acta Source Type: research