Meiotic development initiation in the fungus Podospora anserina requires the peroxisome receptor export machinery

Publication date: Available online 4 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Fernando Suaste-Olmos, Claudia Zirión-Martínez, Harumi Takano-Rojas, Leonardo Peraza-Reyes Peroxisomes are versatile organelles essential for diverse developmental processes. One such process is the meiotic development of Podospora anserina. In this fungus, absence of the docking peroxin PEX13, the RING-finger complex peroxins, or the PTS2 co-receptor PEX20 blocks sexual development before meiocyte formation. However, this defect is not seen in the absence of the receptors PEX5 and PEX7, or of the docking peroxins PEX14 and PEX14/17. Here we describe the function of the remaining uncharacterized P. anserina peroxins predictably involved in peroxisome matrix protein import. We show that PEX8, as well as the peroxins potentially mediating receptor monoubiquitination (PEX4 and PEX22) and membrane dislocation (PEX1, PEX6 and PEX26) are indeed implicated in peroxisome matrix protein import in this fungus. However, we observed that elimination of PEX4 and PEX22 affects to different extent the import of distinct PEX5 cargoes, suggesting differential ubiquitination-complex requirements for the import of distinct proteins. In addition, we found that elimination of PEX1, PEX6 and PEX26 results in loss of peroxisomes, suggesting that these peroxins restrain peroxisome removal in specific physiological conditions. Finally, we demonstrate that all analy...

Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 4, 2018 Category: Molecular Biology Source Type: research