Glucose Transport Activity Measured in Giant Vesicles.
Glucose Transport Activity Measured in Giant Vesicles.
Methods Mol Biol. 2018;1713:77-91
Authors: Hansen JS, Lindkvist-Petersson K
Abstract
Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.
PMID: 29218519 [PubMed - in process]
Source: Molecular Medicine - Category: Molecular Biology Authors: Hansen JS, Lindkvist-Petersson K Tags: Methods Mol Biol Source Type: research
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