Development of novel fluorescent particles applicable for phagocytosis assays with human macrophages.

The objective of this work was the preparation of fluorescent microspheres by a simple method and the evaluation of its applicability in phagocytosis assays by using different human derived cells, differentiated THP-1 cell line and blood monocytes, with flow cytometry measurements for functionality assays. Our results show that microparticles are efficiently internalized in a non-opsonized form and in dose dependent manner by both cellular types. Concerning mechanism we determined that tTG -β3 integrin signaling could be involved in the uptake of these particles.
PMID: 29144198 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - November 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research