Imaging Translation Dynamics of Single mRNA Molecules in Live Cells.

Imaging Translation Dynamics of Single mRNA Molecules in Live Cells.
Methods Mol Biol. 2018;1649:385-404
Authors: Ruijtenberg S, Hoek TA, Yan X, Tanenbaum ME
Abstract
mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe a recently developed microscopy-based method that allows for visualization of translation of single mRNAs in live cells. The ability to measure translation dynamics of single mRNAs will enable a better understanding of spatiotemporal control of translation, and will provide unique insights into translational heterogeneity of different mRNA molecules in single cells.
PMID: 29130212 [PubMed - in process]

Source: Mol Biol Cell - November 14, 2017 Category: Molecular Biology Authors: Ruijtenberg S, Hoek TA, Yan X, Tanenbaum ME Tags: Methods Mol Biol Source Type: research