CryoEM-based hybrid modeling approaches for structure determination.

CryoEM-based hybrid modeling approaches for structure determination.
Curr Opin Microbiol. 2017 Nov 03;43:14-23
Authors: Cassidy CK, Himes BA, Luthey-Schulten Z, Zhang P
Abstract
Recent advances in cryo-electron microscopy (cryoEM) have dramatically improved the resolutions at which vitrified biological specimens can be studied, revealing new structural and mechanistic insights over a broad range of spatial scales. Bolstered by these advances, much effort has been directed toward the development of hybrid modeling methodologies for the construction and refinement of high-fidelity atomistic models from cryoEM data. In this brief review, we will survey the key elements of cryoEM-based hybrid modeling, providing an overview of available computational tools and strategies as well as several recent applications.
PMID: 29107896 [PubMed - as supplied by publisher]

Source: Current Opinion in Microbiology - November 3, 2017 Category: Microbiology Authors: Cassidy CK, Himes BA, Luthey-Schulten Z, Zhang P Tags: Curr Opin Microbiol Source Type: research