Big data in cryoEM: automated collection, processing and accessibility of EM data.

Big data in cryoEM: automated collection, processing and accessibility of EM data.
Curr Opin Microbiol. 2017 Oct 31;43:1-8
Authors: Baldwin PR, Tan YZ, Eng ET, Rice WJ, Noble AJ, Negro CJ, Cianfrocco MA, Potter CS, Carragher B
Abstract
The scope and complexity of cryogenic electron microscopy (cryoEM) data has greatly increased, and will continue to do so, due to recent and ongoing technical breakthroughs that have led to much improved resolutions for macromolecular structures solved using this method. This big data explosion includes single particle data as well as tomographic tilt series, both generally acquired as direct detector movies of ∼10-100 frames per image or per tilt-series. We provide a brief survey of the developments leading to the current status, and describe existing cryoEM pipelines, with an emphasis on the scope of data acquisition, methods for automation, and use of cloud storage and computing.
PMID: 29100109 [PubMed - as supplied by publisher]

Source: Current Opinion in Microbiology - October 31, 2017 Category: Microbiology Authors: Baldwin PR, Tan YZ, Eng ET, Rice WJ, Noble AJ, Negro CJ, Cianfrocco MA, Potter CS, Carragher B Tags: Curr Opin Microbiol Source Type: research