Variability in the response of canine and human dendritic cells stimulated with Brucella canis

This study analysed the cytokine pattern production, by RT-qPCR and ELISA, in human and canine DCs against wholeB. canis or its purified LPS. Human and canine DCs produced different patterns of cytokines after stimulation withB. canis. In particular, while human DCs produced a Th1-pattern of cytokines (IL-1 β, IL-12, and TNF-α), canine cells produced both Th1 and Th17-related cytokines (IL-6, IL-12, IL-17, and IFN-γ). Thus, differences in susceptibility and pathogenicity between these two hosts could be explained, at least partly, by the distinct cytokine patterns observed in this study, where we pr opose that human DCs induce an effective Th1 immune response to control the infection, while canine DCs lead to a less effective immune response, with the activation of Th17-related response ineffective to control theB. canis infection.
Source: Veterinary Research - Category: Veterinary Research Source Type: research