Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria.
Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria.
Methods Mol Biol. 2018;1696:137-145
Authors: Magnuson A, Cardona T
Abstract
The isolation of thylakoid membranes, including intact membrane protein complexes, from heterocysts of filamentous cyanobacteria such as Nostoc punctiforme, is described. Protocols for BN-PAGE/SDS-PAGE 2-D electrophoresis are not included. However, the chapter ends with advisory notes on sample preparation for blue-native PAGE of thylakoid membrane proteins, which can then be used together with any standard protocol.
PMID: 29086401 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Magnuson A, Cardona T Tags: Methods Mol Biol Source Type: research
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