Activity analysis of LTR12C as an effective regulatory element of the RAE1 gene.

We examined the transcriptional activity of LTR12C by using the luciferase assay, and showed that the tandem repeat region (TRR) located within LTR12C was required for its regulatory function. A bioinformatics analysis revealed that the LTR12C element had multiple transcription factor binding sites specific for nuclear transcription factor Y (NF-Y), and the promoter activity of LTR12C was significantly decreased after NF-Y knockdown. Additionally, we discovered novel data indicating that LTR12C was initially inserted into the gorilla genome. Taken together, our results reveal that the TRR of LTR12C has powerful regulatory activity due to its NF-Y binding sites, and the integration of the LTR12C element into the primate genome during evolution may have affected RAE1 transcription.
PMID: 28867566 [PubMed - as supplied by publisher]

Source: Gene - August 31, 2017 Category: Genetics & Stem Cells Authors: Jung YD, Lee HE, Jo A, Hiroo I, Cha HJ, Kim HS Tags: Gene Source Type: research