Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis.

Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis.
BMB Rep. 2017 Jun 12;:
Authors: Jo HN, Kang H, Lee A, Choi J, Chang W, Lee MS, Kim J
Abstract
The Nogo-B receptor (NgBR) is necessary for not only Nogo-B-mediated angiogenesis but also vascular endothelial growth factor (VEGF)-induced angiogenesis. However, the molecular mechanisms underlying the regulatory role of the VEGF-NgBR axis in angiogenesis are not fully understood. Here, we report that miR-26a serves as a critical regulator of VEGF-mediated angiogenesis through directly targeting NgBR in endothelial cells (ECs). Stimulation of ECs by VEGF increased the expression of NgBR and decreased the expression of miR-26a. In addition, miR-26a decreased the VEGF-induced migration and proliferation of ECs. Moreover, miR-26a overexpression in ECs decreased the VEGF-induced phosphorylation of the endothelial nitric oxide synthase (eNOS) and the production of nitric oxide, which is important for angiogenesis. Overall, our data suggest that miR-26a plays a key role in VEGF-mediated angiogenesis through the modulation of eNOS activity, which is mediated by its ability to regulate NgBR expression by directly targeting the NgBR 3'-UTR.
PMID: 28602162 [PubMed - as supplied by publisher]

Source: BMB Reports - June 12, 2017 Category: Biochemistry Authors: Jo HN, Kang H, Lee A, Choi J, Chang W, Lee MS, Kim J Tags: BMB Rep Source Type: research