Enterovirus 71 Inhibits Cellular Type I Interferon Signaling by Downregulating JAK1 Protein Expression

Viral Immunology , Vol. 0, No. 0.
Source: Viral Immunology - Category: Allergy & Immunology Tags: article Source Type: research

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Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Source Type: research
Abstract To characterize the clinical course and outcome of children with status asthmaticus (SA) admitted to a Pediatric Intensive Care Unit (PICU) METHODS: All patients with SA who were admitted to a PICU from January 2003 to December 2018 were reviewed. Polymerase chain reaction (PCR) studies on nasopharyngeal aspirate for respiratory pathogens were performed from 2014 to 2018. RESULTS: Sixty-seven SA admissions constituted 2.4% of total PICU admissions (n=2788). Fifteen (22.4%) children required noninvasive ventilation (NIV) while 7 children (10%) required invasive mechanical ventilation. Nonadherence to prior...
Source: Respiratory Care - Category: Respiratory Medicine Authors: Tags: Clin Respir J Source Type: research
AbstractTo characterize the clinical course and outcome of children with status asthmaticus (SA) admitted to a Pediatric Intensive Care Unit (PICU)MethodsAll patients with SA who were admitted to a PICU from January 2003 to December 2018 were reviewed. Polymerase chain reaction (PCR) studies on nasopharyngeal aspirate for respiratory pathogens were performed from 2014 to 2018.ResultsSixty ‐seven SA admissions constituted 2.4% of total PICU admissions (n=2788). Fifteen (22.4%) children required noninvasive ventilation (NIV) while 7 children (10%) required invasive mechanical ventilation. Nonadherence to prior asthma thera...
Source: The Clinical Respiratory Journal - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLE Source Type: research
In this study, we developed a rapid, simple, sensitive, and specific isothermal reverse transcription assay (RT-Loop -Mediated Amplification, RT-LAMP) for the detection ofEV-A, B, C, and D species of enteroviruses, by targeting the highly conserved 5 ′UTR region. The assay was designed and validated based on reference sequences of the four species and on clinical and environmental isolates. The limit of detection of the assay is 0.75 CCID50/assay forEnterovirus A, B, andD and 0.075 CCID50/assay forEnterovirus C. LAMP allows immediate diagnosis in just 30 –50 min, instead of a minimum of 120 min needed...
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research
WEDNESDAY, Jan. 15, 2020 -- In an observational cohort study recently published in The Lancet Infectious Diseases, details are presented for an outbreak of enterovirus A71 (EV-A71) neurological disease in children who presented to a Colorado...
Source: Drugs.com - Pharma News - Category: Pharmaceuticals Source Type: news
Mu Liu Chi Respiratory viruses are a common cause of respiratory tract infection (RTI), particularly in neonates and children. Rapid and accurate diagnosis of viral infections could improve clinical outcomes and reduce the use of antibiotics and treatment sessions. Advances in diagnostic technology contribute to the accurate detection of viruses. We performed a multiplex real-time polymerase chain reaction (PCR) to investigate the viral etiology in pediatric patients and compared the detection rates with those determined using traditional antigen tests and virus cultures. Fifteen respiratory viruses were included...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
ACS Applied Materials&InterfacesDOI: 10.1021/acsami.9b17412
Source: ACS Applied Materials and Interfaces - Category: Materials Science Authors: Source Type: research
AbstractTo estimate the impact of implementing in-hospital enterovirus (EV) polymerase chain reaction (PCR) testing of cerebrospinal fluid (CSF) with same-day turn-around-time (TAT) on length-of-stay (LOS), antibiotic use and on cost per patient with suspected EV meningitis, compared with testing at an outside reference laboratory. A model-based analysis using a retrospective cohort of all hospitalized children with CSF EV PCR testing done between November 2013 and 2017. The primary outcome measured was the potential date of discharge if the EV PCR result had been available on the same day. Patients with positive EV PCR we...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
In this study, the recombinant nonstructural 2B protein of EV-A71 was successfully produced in human neuroblastoma SH-SY5Y cells and evaluated for its effects on induction of the cell apoptosis and the pathway involved. The EV-A71 2B-transfected SH-SY5Y cells showed significantly higher difference in the cell growth inhibition than the mock and the irrelevant protein controls. The transfected SH-SY5Y cells underwent apoptosis and showed the significant upregulation of caspase-9 (CASP9) and caspase-12 (CASP12) genes at 3- and 24-h post-transfection, respectively. Interestingly, the level of cytosolic Ca2+ was significantly ...
Source: Journal of NeuroVirology - Category: Neurology Source Type: research
Enterovirus 71 (EV-A71) is one of the major causative agents of hand, foot, and mouth disease. EV-A71 infection is sometimes associated with severe neurological diseases such as acute encephalitis, acute flacc...
Source: Journal of Biomedical Science - Category: Biomedical Science Authors: Tags: Review Source Type: research
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