Analysis of honeybush tea (Cyclopia spp.) volatiles by comprehensive two-dimensional gas chromatography using a single-stage thermal modulator.

Analysis of honeybush tea (Cyclopia spp.) volatiles by comprehensive two-dimensional gas chromatography using a single-stage thermal modulator.
Anal Bioanal Chem. 2017 Apr 17;:
Authors: Ntlhokwe G, Tredoux AGJ, Górecki T, Edwards M, Vestner J, Muller M, Erasmus L, Joubert E, Christel Cronje J, de Villiers A
Abstract
The applicability of comprehensive two-dimensional gas chromatography (GC×GC) using a single-stage thermal modulator was explored for the analysis of honeybush tea (Cyclopia spp.) volatile compounds. Headspace solid phase micro-extraction (HS-SPME) was used in combination with GC×GC separation on a non-polar × polar column set with flame ionisation (FID) detection for the analysis of fermented Cyclopia maculata, Cyclopia subternata and Cyclopia genistoides tea infusions of a single harvest season. Method optimisation entailed evaluation of the effects of several experimental parameters on the performance of the modulator, the choice of columns in both dimensions, as well as the HS-SPME extraction fibre. Eighty-four volatile compounds were identified by co-injection of reference standards. Principal component analysis (PCA) showed clear differentiation between the species based on their volatile profiles. Due to the highly reproducible separations obtained using the single-stage thermal modulator, multivariate data analysis was simplified. The results demonstrate both the complexity of honeybush volatile profiles and t...

Source: Analytical and Bioanalytical Chemistry - April 17, 2017 Category: Chemistry Authors: Ntlhokwe G, Tredoux AGJ, Górecki T, Edwards M, Vestner J, Muller M, Erasmus L, Joubert E, Christel Cronje J, de Villiers A Tags: Anal Bioanal Chem Source Type: research