Extraction and isolation of potential anti-stroke compounds from flowers of Pueraria lobata guided by in vitro PC12 cell model.

Extraction and isolation of potential anti-stroke compounds from flowers of Pueraria lobata guided by in vitro PC12 cell model.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 24;1048:111-120
Authors: Li S, Li S, Liu C, Liu C, Zhang Y
Abstract
A simple and efficient method based on ultrafiltration liquid chromatography-mass spectrometry (UFLC-MS) was applied to rapidly screen and identify ligands for lactate dehydrogenase (LDH) from the flowers of Pueraria lobata, and the compounds were assessed for anti-stroke activity using a PC12 cell model. Seven major isoflavones, kakkalide, 3'-hydroxy puerarin, puerarin, puerarin xyloside, tectoridin, tectorigenin, and ononin, were identified as potent LDH inhibitors. A continuous online method, which consisted of microwave-assisted extraction and countercurrent chromatography (MAE-CCC), was newly developed for scaled-up production of these compounds with high purity and efficiency. This novel approach, using UFLC-MS coupled with MAE-CCC and a PC12 cell model, provided a powerful tool for screening, extraction, and separation of LDH inhibitors from complex samples, and a useful platform for the large-scale production of functional food and nutraceutical ingredients.
PMID: 28236683 [PubMed - indexed for MEDLINE]

Source: Anal Sci - March 17, 2017 Category: Chemistry Authors: Li S, Li S, Liu C, Liu C, Zhang Y Tags: J Chromatogr B Analyt Technol Biomed Life Sci Source Type: research