Ferrocenyl naphthalene diimides as tetraplex DNA binders.

Ferrocenyl naphthalene diimides as tetraplex DNA binders.
J Inorg Biochem. 2016 Nov 17;167:21-26
Authors: Sato S, Takenaka S
Abstract
Seven ferrocenyl naphthalene diimide (FND) ligands were synthesized. Each had a higher affinity for tetraplex DNA than for either single- or double-stranded DNA. The FND binding affinities were >10(5)M(-1) in 0.10M AcOH-AcONa or AcOH-AcOK (pH5.5) containing 0.10M NaCl or KCl. The FNDs with the highest binding affinities for tetraplex DNA showed 23- or 8-times higher preference for tetraplex DNA than for single- or double-stranded DNA, respectively. The current signals generated from the seven FNDs bound to the tetraplex DNA immobilized on the electrode were found to correlate with the binding affinities of these ligands for the tetraplex DNA. Furthermore, using the telomerase repeat amplification protocol assay, the FND ligands could be categorized into three groups: (a) inhibiting both telomerase and Taq polymerase, (b) inhibiting telomerase alone, and (c) inhibiting neither telomerase nor Taq polymerase.
PMID: 27893990 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - November 16, 2016 Category: Biochemistry Authors: Sato S, Takenaka S Tags: J Inorg Biochem Source Type: research