Protocol for the Measurement of Hydrocarbon Transport in Bacteria

Due to the hydrophobicity, volatility, and relatively low aqueous solubility of aliphatic and aromatic hydrocarbons, transport of these chemicals by bacteria has not been extensively studied. These issues make transport assays difficult to carry out, and as a result, strong evidence for the active transport of hydrocarbons is lacking. Here we describe a detailed protocol for the measurement of hydrocarbon transport in bacteria and suggest key equipment and control experiments required to obtain convincing results.

Source: Springer protocols feed by Microbiology - November 16, 2016 Category: Microbiology Source Type: news