Establishment of Anti-Human ATRX Monoclonal Antibody AMab-6.

In this study, we immunized mice with recombinant human ATRX and developed a novel mAb, AMab-6. The dissociation constant of AMab-6 was determined to be 9.7 × 10(-10) M, indicating that the binding affinity of AMab-6 is very high. Furthermore, AMab-6 sensitively detects ATRX in Western blot and immunohistochemical analyses, indicating that AMab-6 could become the standard marker to determine the ATRX mutation status of gliomas in immunohistochemical analyses.
PMID: 27788029 [PubMed - in process]

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 29, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research