The Replicative Detargeting FISH (ReD-FISH) Technique in Studies of Telomere Replication

Based on the chromosome orientation-FISH (CO-FISH) procedure, the replicative detargeting FISH (ReD-FISH) was developed as a unique tool to study the replicative patterns of telomeres located on individual chromosomal arms. This method is also suited for examination of telomeres of species belonging to different classes of animals, for which well-proliferated cell cultures can be established and maintained. ReD-FISH is based on pulsed inclusion of labeled brominated nucleotides in replicating DNA, destruction of regions with brominated analogs, and standard FISH with single-strand-specific probe/probes.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news