Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells.

Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells. Biomed Environ Sci. 2016 Jul;29(7):524-528 Authors: Zhang PW, Tian C, Xu FY, Chen Z, Burnside R, Yi WJ, Xiang SY, Xie X, Wu NN, Yang H, Zhao NN, Ye XL, Ying CJ Abstract Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia. PMID: 27554123 [PubMed - as supplied by publisher]
Source: Biomedical and Environmental Sciences : BES - Category: Biomedical Science Authors: Tags: Biomed Environ Sci Source Type: research