Development of immunosensors based on optical waveguide lightmode spectroscopy (OWLS) technique for determining active substance in herbs

Publication date: February 2017 Source:Sensors and Actuators B: Chemical, Volume 239 Author(s): Nóra Adányi, Krisztina Majer-Baranyi, Mária Berki, Béla Darvas, Baomin Wang, István Szendrő, András Székács For the quick and reliable quantification of special active substances derived from herbs, a new type of immunosensor based on optical waveguide lightmode spectroscopy (OWLS) detection was investigated. Artemisinin, an antimalarial drug derived from the sweet wormwood plant Artemisia annua is a sesquiterpene lactone endoperoxide, and it is distilled from the dried leaves or flower clusters of A. annua. Numerous derivatives of artemisinin, including artesunate and artemether, are also being used in the treatment of malaria, and these compounds have recently gained utility also as anticancer agents. To quantitatively determine the presence of these biologically effective substances in various herbs, a novel OWLS-based immunosensor was investigated. To create regenerable sensitized surfaces in the OWLS technique so that the sensor can be applied several times, the antigen or the antibody were immobilized by covalent attachment to the silanized surfaces of the OWLS chips. When measuring with the antibody capture mode antibodies raised against the appropriate artemisinin derivative (artemisinin, artesunate or artemether) were immobilized on the sensor surface and the linear measuring range was determined. During the antigen capture measurement the protein conjuga...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research