Inaccurate quantitation of palmitate in metabolomics and isotope tracer studies due to plastics

ConclusionWhen measuring palmitate standard solutions, the best results were obtained when glass vials and glass pipettes were used. However, much of the palmitate background signal could be eliminated by pre-rinsing plastic vials and plastic pipette tips with methanol prior to sample introduction. For isotope tracer studies, error could also be minimized by estimating palmitate enrichment from palmitoylcarnitine, which does not have a competing contamination signal from plastic consumables.

Source: Metabolomics - August 7, 2016 Category: Biology Source Type: research