Structural studies of the formation of lipoplexes between siRNA and selected bis-imidazolium gemini surfactants.

In this study, we demonstrated that bis-imidazolium gemini surfactants with variable lengths of dioxyalkyl linker groups (from dioxyethyl to dioxydodecyl) and dodecyl side chains are excellent for the complexation of siRNA. All of these compounds effectively complexed siRNA in a charge ratio range (p/n) of 1.5-10. The low resolution structure of siRNA oligomers was characterised by small angle scattering of synchrotron radiation (SR-SAXS) and ab initio modelling. The structures of the formed complexes were also analysed using SR-SAXS, circular dichroism studies and electrophoretic mobility tests. The most promising agents for complexation with siRNA were the surfactants that contained dioxyethyl and dioxyhexyl spacer groups.
PMID: 27424091 [PubMed - as supplied by publisher]

Source: Colloids and Surfaces - June 28, 2016 Category: Biotechnology Authors: Andrzejewska W, Pietralik Z, Skupin M, Kozak M Tags: Colloids Surf B Biointerfaces Source Type: research