Evaluation of a time efficient immunization strategy for anti-PAH antibody development.

Evaluation of a time efficient immunization strategy for anti-PAH antibody development.
J Immunoassay Immunochem. 2016 Jun 9;
Authors: Li X, Kaattari SL, Vogelbein MA, Unger MA
Abstract
The development of monoclonal antibodies (mAb) with affinity to small molecules can be a time-consuming process. To evaluate shortening the time for mAb production, we examined mouse antisera at different time points post-immunization to measure titer and to evaluate the affinity to the immunogen PBA (pyrene butyric acid). Fusions were also conducted temporally to evaluate antibody production success at various time periods. We produced anti-PBA antibodies 7 weeks post-immunization and selected for anti-PAH reactivity during the hybridoma screening process. Moreover, there were no obvious sensitivity differences relative to antibodies screened from a more traditional 18 week schedule. Our results demonstrate a more time efficient immunization strategy for anti-PAH antibody development that may be applied to other small molecules.
PMID: 27282486 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - June 11, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research