Cytoplasmic DNA disclose high nucleotide diversity and different phylogenetic pattern in Taihangia rupestris Yu et Li

Publication date: June 2016 Source:Biochemical Systematics and Ecology, Volume 66 Author(s): Yueqin Cheng, Jingmian Duan, Zhenbin Jiao, G. Geoff Wang, Fengming Yan, Hongwei Wang Taihangia rupestris Yu et Li is an ancient species endemic to the Taihang Mountains, where it originated and evolved. Six chloroplast DNA (cpDNA) (trnT UGU -trnL UAA 5′exon, trnL UAA 5′exon-3′exon, trnL UAA 3′exon-trnF GAA , trnH GUG -trnK UUU , psbC-trnS and rps12-rpl20) and five mitochondrial DNA regions (rrn5/rrn18-2, nad1/2-3, nad4/3-4, nad7/1-2 and nad7/2-3) were employed to investigate the nucleotide diversity and subspecies differentiation. The results showed that T. rupestris had high nucleotide diversity at the species level, and T. rupestris var. ciliate possessed higher levels of haplotype and nucleotide diversity than did T. rupestris var. rupestris. Cytoplasmic DNA revealed a phylogenetic pattern of individuals that was in disagreement with the patterns obtained from nuclear DNA. These results primarily reflect the past habitat fragmentation of the species and different modes of inheritance and spread of these genomes. Together, our results advance our understanding of plant speciation in this region.

Source: Biochemical Systematics and Ecology - May 18, 2016 Category: Biochemistry Source Type: research