Fluorescent Formazans and Tetrazolium Salts - Towards Fluorescent Cytotoxicity Assays.
Fluorescent Formazans and Tetrazolium Salts - Towards Fluorescent Cytotoxicity Assays. Comb Chem High Throughput Screen. 2016 Apr 8; Authors: Ladyman MK, Walton JG, Lilienkampf A, Bradley M Abstract Formazan-based colorimetric cytotoxicity assays, such as the MTT assay, are typically used to assess cell viability with only metabolically active cells reducing tetrazolium salts into the formazans, which is then quantified by absorbance. Fluorescence offers several advantages compared to colorimetric assays and would enable techniques such as flow cytometry and confocal microscopy to be used for analysis. Here, fluorescent formazans 10, 11 and 12, and their corresponding tetrazolium salts 13, 16 and 24, respectively, were synthesised by incorporation of a known fluorophore backbone (coumarin, fluorescein and rhodol) with disruption of the conjugated system preventing or reducing fluorescence of the tetrazolium salts. The tetrazolium moiety was able to quench the fluorescence of the incorporated fluorescein and O-methyl rhodol, whereas with the coumarin-based tetrazolium salt the fluorescence was only quenched under acidic conditions. These tetrazolium salts were successfully reduced to the fluorescent formazans with cells and offer a step forward in the development of fluorescent cytotoxicity assays. PMID: 27055750 [PubMed - as supplied by publisher]
Researchers use non-toxic silicon nanocrystals to get closer to photodynamic cancer treatment More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=299685&WT.mc_id=USNSF_1 This is a Research News item.
Research leads to clearer picture of the plague’s effects More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=299687&WT.mc_id=USNSF_1 This is a Research News item.
New research investigates what proportion of the world's land plants are exceedingly rare More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=299688&WT.mc_id=USNSF_1 This is a Research News item.
Dec 12 2019 8:30AM toDec 13 2019 1:00PMRoom E2020Fall 2019 Advisory Committee (AC) Meeting to the Directorate for Social, Behavioral &Economic Sciences (SBE) More at https://www.nsf.gov/events/event_summ.jsp?cntn_id=297395&WT.mc_id=USNSF_13&WT.mc_ev=click This is an NSF Events item.
Dec 16 2019 3:00PM toDec 16 2019 4:00PMNational Science Foundation, 2415 Eisenhower Avenue, Alexandria, VA 22314See attached notice for details. More at https://www.nsf.gov/events/event_summ.jsp?cntn_id=299690&WT.mc_id=USNSF_13&WT.mc_ev=click This is an NSF Events item.
Available Formats: HTML: https://www.nsf.gov/pubs/2020/nsf20025/nsf20025.jsp?WT.mc_id=USNSF_25&WT.mc_ev=clickPDF: https://www.nsf.gov/pubs/2020/nsf20025/nsf20025.pdf?WT.mc_id=USNSF_25&WT.mc_ev=clickDocument Number: nsf20025This is an NSF Program Announcements and Information item.
Publication date: Available online 9 December 2019Source: MethodsXAuthor(s): Fatemeh Tamaddon, Alireza Nasiri, Ghazal YazdanpanahAbstractHerein, magnetically separable CuFe2O4@methyl cellulose (MC) as a novel magnetic nanobiocomposite photocatalyst was synthesized with a facile, rapid, green, and new microwave-assisted method. After that, CuFe2O4@MC was characterized with FESEM, EDS, FT-IR, XRD, TGA, and VSM techniques. To measure CuFe2O4@MC photocatalytic activity, ciprofloxacin (CIP) removal ability of CuFe2O4@MC was investigated under the conditions such as initial CIP concentrations (3, 5, 7, and 9 mg/L), pHs (3...
Robotic junk collector will be first mission to remove item of debris from orbitA four-armed robotic junk collector will be launched into space by the European Space Agency in what it says will be the first mission to remove an item of debris from orbit.The ClearSpace-1 mission, scheduled for launch in 2025, will cost €120m and will grab a single piece of junk. But the agency hopes the mission will pave the way for a wide-reaching clear-up operation, with Esa’s director general calling for new rules that would compel those who launch satellites to take responsibility for removing them from orbit once they are re...
Publication date: Available online 9 December 2019Source: Journal of Environmental SciencesAuthor(s): Yun Chen, Da Ouyang, Wenying Zhang, Jingchun Yan, Linbo Qian, Lu Han, Mengfang ChenAbstractA novel insight on the role of interactions between target pollutants and the catalyst in the copper-containing layered double oxide (LDO)-catalyzed persulfate (PS) system was elucidated in the present study. 4-Chlorophenol (4-CP), as a representative benzene derivative with a hydroxyl group, was completely removed within 5 min, which was much faster than the reaction of monochlorobenzene (MCB), without a hydroxyl group, with th...
In this study, biofilms characteristics of aerobic rural SDGS were investigated using simulations in a lab under different flow conditions and slopes. Results indicated that the average thickness of aerobic rural SDGS biofilms was in the range of 350–650 μm, decreasing at locations with variable flow and high slopes. Protein was the most abundant substance in extracellular polymeric substance of SDGS biofilms. The most abundant bacteria, Proteobacteria, Actinobacteria, and Bacteroidetes, and functional bacteria showed different distributions when analyzed through Illumina HiSeq sequencing of 16S rRNA. The rel...
More News: Chemistry