Inhibition of Quorum Sensing-Controlled Virulence Factors in Pseudomonas aeruginosa by Human Serum Paraoxonase (hPON1).

Inhibition of Quorum Sensing-Controlled Virulence Factors in Pseudomonas aeruginosa by Human Serum Paraoxonase (hPON1).
J Med Microbiol. 2015 Dec 9;
Authors: Aybey A, Demirkan E
Abstract
The role of quorum sensing (QS) on the regulation of virulence factor production in Pseudomonas aeruginosa is well established. Increased antibiotic resistance in this bacterium has led to the search for new treatment options, and inhibition of the QS system has been explored for potential therapeutic benefits. If the use of QS inhibitory agents were to lead to a reduction in bacterial virulence, new approaches in the treatment of P. aeruginosa infections could be further developed. Accordingly, we examined whether human serum paraoxonase 1 (hPON1), which uses lactonase activity to hydrolyze acyl homoserine lactones (AHLs), could cleave P. aeruginosa-derived signaling molecules. hPON1 was purified using ammonium sulfate precipitation and hydrophobic interaction chromatography (Sepharose 4B-L-tyrosine-1-naphthylamine). Different concentrations of hPON1 have been found to reduce various virulence factors including pyocyanin, rhamnolipid, elastase, staphylolytic LasA protease and alkaline protease. Although treatment with 0,1-10 mg∙mL-1 hPON1 did not show a highly inhibitory effect on elastase and staphylolytic LasA protease production, it resulted in good inhibitory effects on alkaline protease production at concentrations as low as 0,1 mg∙mL-1. hP...

Source: Journal of Medical Microbiology - December 9, 2015 Category: Microbiology Authors: Aybey A, Demirkan E Tags: J Med Microbiol Source Type: research