Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
We describe a semi-automated and simple method for the accurate and precise measurement of PRA from 500 μL of plasma (250 μL if blank subtraction is omitted, as discussed) using a liquid chromatography and tandem mass spectrometry (LC-MS/MS) method for angiotensin I (AngI) in 96-well format. After a 3 h AngI generation step at 37 °C in buffering conditions at pH 6, the reaction is quenched with 10 % formic acid containing AngI internal standard. Sample preparation then proceeds with offline solid phase extraction, two wash steps, and methanol elution followed by injection into the LC-MS/MS system. Quantitation is performed against a 7-point calibration linear curve prepared in buffer. The assay calibration range is 0.34–30.0 ng/mL which corresponds to PRA values of 0.11–10.0 ng/mL/h: much wider than was possible using traditional competitive antibody-based methods. Total precision in clinical production has been observed to be 5.8 to 5.0 % for Bio-Rad Hypertension Control materials having nominal PRA values ranging from 1.73 to 12.43 ng/mL/h. At AngI concentrations of 0.06 ng/L (corresponding to a PRA of 0.02 ng/mL/h), signal to noise ratios are 50:1 indicating that the limit of quantitation is well below the level required for clinical use.
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Conclusions: In our large cohort of CAS patients, coexistent UIAs are not uncommon. Stenting of a carotid artery in the presence of coexistent UIAs could be conducted safely. Together with 3-month dual antiplatelet therapy, CAS did not increase the rupture risk of the coexistent UIAs within 3 months.Cerebrovasc Dis
This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence.Taslim A. Al-Hilal, Ali Keshavarz, Hossam Kadry, Behnaz Lahooti, Ahmed Al-Obaida, Zhenya Ding, Wei Li, Roger Kamm, Ivan F. McMurtry, Tim Lahm, Eva Nozik-Grayck, Kurt R. Stenmark, Fakhrul Ahsan Current animal and cellular models only partially recapitulate the pathophysiology of PAH. Our PAH-on-a-chip model can mimic the major pathologies of PAH such as pulmonary arterial remodeling and sex disparity and help develop PAH therapeutics. To cite this article before page numbers are assigned, use the DOI form of citation above. The c...
CONCLUSION: Kidney biopsy should be considered an important diagnostic tool in this clinical scenario. ABBREVIATIONS: ACE: angiotensin-converting enzyme; ESKD: end-stage kidney disease; FSGS: focal segmental glomerulosclerosis; RAAS: renin aldosterone angiotensin system. PMID: 32744913 [PubMed - as supplied by publisher]
CONCLUSION: Aberrant circadian pattern of BP in women with PE with severe features was associated with several adverse maternal/perinatal outcomes. PMID: 32744911 [PubMed - as supplied by publisher]
Fluid overload is a serious issue for critically ill patients. It can occur in a variety of situations, including as a result of acute kidney injury, and can also be an issue in critically ill COVID-19 patients. The condition requires prompt treatmen...
Abstract CONTEXT: Patients with adrenal insufficiency (AI) (primary [PAI], secondary to pituitary disease [PIT] and congenital adrenal hyperplasia [CAH]) have reduced life expectancy; however, the underlying explanation remains unknown. OBJECTIVE: To evaluate characteristics, comorbidities and hospitalizations in AI patients. DESIGN: Retrospective Observational Setting and Population: Using a US-based national payer database comprising>108 million members, strict inclusion criteria including diagnostic codes and steroid prescription records were used to identify 10,383 adults with AI; 1,014 with PAI, ...