Simultaneous determination of ursolic acid and eugenol from Ocimum sanctum L. cultivated by organic and non-organic farming

Title: Simultaneous determination of ursolic acid and eugenol from Ocimum sanctum L. cultivated by organic and non-organic farming

Authors: Itankar, Prakash R; Tauqeer, Mohammad; Dalal, Jayshree S; Chatole, Pranali G

Abstract: Worldwide revolution for the improvement of people safety is gaining
momentum; hence drug safety for the subject becomes even more prominent in the
present day scenario. Sothat, organic farming becomes a topic of general interest all
over the globe. In the present study an attempt has been made to develop a simple, precise,
rapid and cost-effective high performance thin layer chromatographic (HPTLC)
method for simultaneous quantitative estimation of ursolic acid and eugenol in
organically and non-organically cultivated Ocimum sanctum L..
The aqueous extracts of organically
(OOS) & non-organically (NOS) cultivated Ocimum sanctum were
investigated for its phyto-constituents using HPTLC studies. Linear regression
data for the calibration curves of standards, viz. ursolic acid and eugenol
showed a good linear relationship over a concentration range of 0.2-1.4
µg/spot, 1.0-7.0 µg/spot respectively with the correlation coefficient of
0.99909 and 0.99914 respectively and thus exhibits good linearity between concentration and
area. The Ocimum sanctum
(OOS) confirmed its better quality with reference to its higher contents of
ursolic acid (0.17) and eugenol (1.77).
The proposed HPTLC method was found to be rapid, simple and linear for
qu...

Source: Indian Journal of Traditional Knowledge - September 28, 2015 Category: Science Authors: search Source Type: research