Antioxidant capacity determination of complex samples and individual phenolics - Multilateral approach.

Antioxidant capacity determination of complex samples and individual phenolics - Multilateral approach.
Comb Chem High Throughput Screen. 2015 Nov 1;
Authors: Petrović M, Sužnjević D, Pastor F, Veljović M, Pezo L, Antić M, Gorjanović S
Abstract
Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP), scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on decrease of anodic current of HydroxoPerhydroxoMercury(II) complex (HPMC) formation in alkaline solution of H2O2, at potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus volume of gradually added complex samples or amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Correlations between applied assays were calculated by regression analysis. Relative antioxidant capacity index (RACI), calculated by assigning equal weight to all applied assays and phenolic antioxidant coefficients (PAC), calculated as ratio...

Source: Combinatorial Chemistry and High Throughput Screening - November 1, 2015 Category: Chemistry Authors: Petrović M, Sužnjević D, Pastor F, Veljović M, Pezo L, Antić M, Gorjanović S Tags: Comb Chem High Throughput Screen Source Type: research