Membrane display and functional analysis of juxtacrine ligand-receptor signaling.

Membrane display and functional analysis of juxtacrine ligand-receptor signaling.
Biotechniques. 2015;59(4):231-40
Authors: Nair PM, Ngu H, Torres E, Marsters S, Lawrence DA, Stephan JP, Komuves L, Ashkenazi A
Abstract
We developed a strategy for identifying modulators of juxtacrine signaling, triggered by a cell-surface ligand displayed on synthetic lipid bilayers, via cognate receptors on apposed cells. Using readouts for receptor lateral transport and intracellular signaling, we screened a small interfering RNA (siRNA) library and identified specific receptor tyrosine kinases (RTKs) that directly or indirectly modulate apoptosis signaling by a model death ligand through its cognate death receptors. This approach may be broadly useful for studying juxtacrine cell-cell signaling systems.
PMID: 26458551 [PubMed - in process]

Source: BioTechniques - October 17, 2015 Category: Biotechnology Authors: Nair PM, Ngu H, Torres E, Marsters S, Lawrence DA, Stephan JP, Komuves L, Ashkenazi A Tags: Biotechniques Source Type: research