A novel sex-linked microsatellite marker for molecular sexing in rock bream fish Oplegnathus fasciatus

Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Dongdong Xu, Bao Lou, Sanli Li, Xinxu Sun, Wei Zhan, Ruiyi Chen, Guomin Mao Sex-specific DNA markers can serve as efficient tools for molecular sex identification and thus provide important information for ecological and evolutionary studies, as well as for fishery management. In the present study, microsatellite markers were employed to identify sex-linked markers in the rock bream (Oplegnathus fasciatus). A microsatellite marker, designated as Oplfa16, displayed a male-specific genotype in rock bream. The male-specific microsatellite marker was further tested in 82 individuals, ensuring reproducible sex identification. Therefore, we developed a rapid and reliable method for sex identification in rock bream by using a novel sex-linked microsatellite marker.

Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research