Haematologic effects of leukoreduction on canine whole blood post-filtration and post-storage

Abstract
Leukoreduction is the removal of leukocytes from a blood component to avoid the adverse reactions resulting from the exposure of leukocytes from a donor to the recipient during transfusion. A leukoreduced blood component has been defined as achieving a concentration of less than 5 × 106 residual donor leukocytes per final blood product while maintaining 85 % of the original red blood cell count. In the present study, we evaluated the efficiency of leukocyte filtration using a BioR 01-plus blood filter (Fresenius Hemocare, Germany) on samples of whole blood in citrate-phosphate-dextrose-adenine (CPDA-1) anticoagulant from 21 dogs. The blood cell count, red blood cells (RBCs), white blood cells (WBCs) and platelets; haemoglobin concentration; packed cell volume (PCV); mean corpuscular volume (MCV); RBC distribution width (RDW); morphologic index (MI) and RBC morphology were evaluated pre-filtration, post-filtration and after storage for 28 days at 4 °C. After filtration, a significant reduction in leukocyte (98 %) and platelet (95.1 %) counts were observed, while the RBCs, haemoglobin concentration and PCV remained within the recommended levels; storage did not change these parameters.

Source: Comparative Clinical Pathology - July 11, 2015 Category: Pathology Source Type: research