Notable enhancement of Amphotericin B channel activity by applied pressures in the range of MS channel activation

Biochim Biophys Acta Biomembr. 2024 May 3;1866(5):184326. doi: 10.1016/j.bbamem.2024.184326. Online ahead of print.ABSTRACTThe mechanism of Amphotericin B at the membrane is still subject of debate, with the prevailing hypothesis being the formation of pores. The activity of these pores is influenced by various factors. Recently aggregation in solution and insertion in the membrane had been highlighted as crucial for action of the drug Here we investigated the effect of applied pressure on the activity of Amphotericin B. Our findings demonstrate that applied pressure of 50 mmHg is sufficient to enhance the activity. We interpreted the results as supporting the idea that pressure fractures the membrane and promotes the insertion of the polyene.PMID:38703804 | DOI:10.1016/j.bbamem.2024.184326

Source: Biochimica et Biophysica Acta - May 4, 2024 Category: Biochemistry Authors: Tammy Haro-Reyes Iv án Ortega-Blake Source Type: research