Simulation of nanopore sequencing signal data with tunable parameters [METHOD]

We present a brief example of Squigulator's use, creating simulated data to model the degree to which different parameters impact the accuracy of ONT basecalling and downstream variant detection. This analysis reveals new insights into the nature of ONT data and basecalling algorithms. We provide Squigulator as an open-source tool for the nanopore community.
Source: Genome Research - Category: Genetics & Stem Cells Authors: Tags: METHOD Source Type: research