Viruses, Vol. 16, Pages 721: Epigenetic Modifications of White Blood Cell DNA Caused by Transient Fetal Infection with Bovine Viral Diarrhea Virus

Viruses, Vol. 16, Pages 721: Epigenetic Modifications of White Blood Cell DNA Caused by Transient Fetal Infection with Bovine Viral Diarrhea Virus
Viruses doi: 10.3390/v16050721
Authors:
Hana Van Campen
Jeanette V. Bishop
Zella Brink
Terry E. Engle
Carolina L. Gonzalez-Berrios
Hanah M. Georges
Jessica N. Kincade
Dilyara A. Murtazina
Thomas R. Hansen

Bovine viral diarrhea virus (BVDV) infections cause USD 1.5–2 billion in losses annually. Maternal BVDV after 150 days of gestation causes transient fetal infection (TI) in which the fetal immune response clears the virus. The impact of fetal TI BVDV infections on postnatal growth and white blood cell (WBC) methylome as an index of epigenetic modifications was examined by inoculating pregnant heifers with noncytopathic type 2 BVDV or media (sham-inoculated controls) on Day 175 of gestation to generate TI (n = 11) and control heifer calves (n = 12). Fetal infection in TI calves was confirmed by virus-neutralizing antibody titers at birth and control calves were seronegative. Both control and TI calves were negative for BVDV RNA in WBCs by RT-PCR. The mean weight of the TI calves was less than that of the controls (p < 0.05). DNA methyl seq analysis of WBC DNA demonstrated 2349 differentially methylated cytosines (p ≤ 0.05) including 1277 hypomethylated cytosines, 1072 hypermethylated cytosines, 84 differentially methylated regions based on CpGs in promoters, and 89 DMRs in...

Source: Viruses - May 1, 2024 Category: Virology Authors: Hana Van Campen Jeanette V. Bishop Zella Brink Terry E. Engle Carolina L. Gonzalez-Berrios Hanah M. Georges Jessica N. Kincade Dilyara A. Murtazina Thomas R. Hansen Tags: Article Source Type: research