VPg-based bidirectional synthetic mRNA circuits enable orthogonal protein regulation for high-resolution cell separation

Chem Commun (Camb). 2024 Apr 30. doi: 10.1039/d4cc01725k. Online ahead of print.ABSTRACTSynthetic mRNA circuits commonly sense input to produce binary output signals for cell separation. Based on virus-origin cap-independent translation initiation machinery and RBP-aptamer interaction, we designed smart synthetic mRNA-based circuits that sense single input molecules to bidirectionally tune output signals in an orthogonal manner, enabling high-resolution separation of cell populations.PMID:38685869 | DOI:10.1039/d4cc01725k

Source: Chemical Communications - April 30, 2024 Category: Chemistry Authors: Zhenghua Liang Yaxin Hu Cheuk Yin Li Wai Laam Yau Kaixin Tan Yi Kuang Source Type: research