A Comparative Study of the Effects of Different Crosslinking Methods on the Physicochemical Properties of Collagen Multifilament Bundles

This study compares different physical (ultraviolet light, UVC) and chemical (genipin, carbodiimide (EDC), and glutaraldehyde) crosslinking methods on contact-drawn collagen multifilament bundles designed to recapitulate the structure of a tendon. The presence of collagen and the formation of well-ordered collagen structures are confirmed by attenuated total reflectance Fourier-transform infrared spectromicroscopy and wide-angle X-ray scattering for all crosslinking methods. The morphology of the collagen multifilament bundles is similar across crosslinking methods. Swelling of the multifilament bundles is dramatically reduced following crosslinking and varies by crosslinking method, with genipin- and carbodiimide-crosslinked specimens swelling the least. Ultimate tensile strength (UTS) and Young's modulus significantly improve for all crosslinked specimens compared to non-crosslinked specimens. Glutaraldehyde crosslinked collagen multifilament bundles display the highest UTS values ranging from 33.82±0.0 MPa to 45.528±0.757 MPa.PMID:38662530 | DOI:10.1002/cphc.202400259

Source: Chemphyschem - April 25, 2024 Category: Chemistry Authors: Rahimeh Rasouli Hessameddin Yaghoobi John Paul Frampton Source Type: research