Chemical control of CRISPR/Cpf1 editing < em > via < /em > orthogonal activation and deactivation of crosslinked crRNA

Chem Commun (Camb). 2024 Apr 23. doi: 10.1039/d4cc01106f. Online ahead of print.ABSTRACTThrough the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.PMID:38651297 | DOI:10.1039/d4cc01106f

Source: Chemical Communications - April 23, 2024 Category: Chemistry Authors: Cui-Lian Lin Wen-Da Chen Li Liu Liang Cheng Source Type: research