A structure-based computational model of IP < sub > 3 < /sub > R1 incorporating Ca and IP3 regulation

In this study we present a coarse-grained model of IP3R1 which accounts for the channel architecture and the location of specific Ca and IP3 binding sites. This computational model accounts for the domain-domain interaction within and between the four subunits that form IP3R1, and also describes how ligand binding regulates these interactions. Using a kinetic model, we explore how two Ca binding sites on the cytosolic side of the channel interact with the IP3 binding site to regulate the channel open probability. Our primary finding is that the bell-shaped open probability of IP3R1 provides constraints on the relative strength of these regulatory binding sites. In particular, we argue that a specific Ca binding site, whose function has not yet been established, is very likely a channel antagonist. Additionally, we apply our model to show that domain-domain interactions between neighboring subunits exert control over channel cooperativity and dictate the nonlinear response of the channel to Ca concentration. This suggests that specific domain-domain interactions play a pivotal role in maintaining the channel's stability, and a disruption of these interactions may underlie disease states associated with Ca dysregulation.PMID:38627970 | DOI:10.1016/j.bpj.2024.04.014

Source: Biophysical Journal - April 17, 2024 Category: Physics Authors: D'Artagnan Greene Yohannes Shiferaw Source Type: research