Label/immobilization-free Cas12a-based electrochemiluminescence biosensor for sensitive DNA detection

Talanta. 2024 Apr 15;275:126114. doi: 10.1016/j.talanta.2024.126114. Online ahead of print.ABSTRACTElectrochemiluminescence (ECL) is one of the most sensitive techniques in the field of diagnostics. However, they typically require luminescent labeling and electrode surface biological modification, which is a time-consuming and laborious process involving multiple steps and may also lead to low reaction efficiency. Fabricating label/modification-free biosensors has become one of the most attractive parts for simplifying the ECL assays. In this work, the ECL luminophores carbon dots (CDs) were encapsulated in DNA hydrogel in situ by a simple rolling circle amplification (RCA) reaction. Upon binding of the target DNA, active Cas12a induces a collateral cleavage of the hydrogel's ssDNA backbone, resulting in a programmable degradation of the hydrogel and the release of CDs. By directly measuring the released CDs ECL, a simple and rapid label/modification-free detection of the target HPV-16 was realized. It is noted that this method allowed for 0.63 pM HPV-16 DNA detection without any amplification step, and it could take only ∼60 min for a fast test of a human serum sample. These results showed that our label/modification-free ECL biosensor has great potential for use in simple, rapid, and sensitive point-of-care (POC) detection.PMID:38631265 | DOI:10.1016/j.talanta.2024.126114

Source: Talanta - April 17, 2024 Category: Chemistry Authors: Honghong Wang Xiaomin Hang Huiyi Wang Jiaxin Peng Haoming Yu Li Wang Source Type: research