Chemical Synthesis of Secretoglobin 3A2 Covalent Homodimer and Photocaged Monomeric Variants

In this report, we document the chemical synthesis of SCGB3A2 in multi-milligram quantities. Key to access both monomeric and homo-dimeric SCGB3A2 analogues was the use of KAHA ligation and enabled masking of the cysteine residue. The synthetic proteins were used to investigate SCGB3A2 self-assembly profile, confirming their high propensity to dimerization even in the absence of the key Cys residue.PMID:38635000 | DOI:10.1002/anie.202404992

Source: Angewandte Chemie - April 18, 2024 Category: Chemistry Authors: Thais Gazzi Maria Heinke Fabienne Landolt Jeffrey W Bode Source Type: research